Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр животноводства – ВИЖ имени академика Л.К. Эрнста»
ABSTRACT. Since commercial media developed for the cultivation of human embryos are pre-tested on mouse embryos, then, according to the author's working hypothesis, there is a potential opportunity to conduct this stage also for other mammalian species, in particular for embryos of cattle or pigs. Productive animals have certain advantages for modeling the development of human embryos, since the reproductive physiology of these animals has been studied quite well, their reproductive organs are available in large quantities at a low price, and both surgical and non-surgical techniques have been developed for the collection of embryos. The lifespan of most productive species is closer to that of humans (compared to rodents as model animals), which may be especially important when attempting to study the effects of age on gametes and the viability of embryos on model animals. Environments for the cultivation of gametes of cattle and pigs are as yet being prepared individually in each laboratory, which significantly reduces the reproducibility of the methods and experimental data. The use of ready-made commercial media would avoid the negative impact of this factor. In this paper, the possibility of maturating pigs oocyte in vitro in a commercial medium of the company Life Global, developed for the cultivation of human embryos, has been studied. The criterion for successful maturation was the presence of the first orienting calf (stage MII), as well as the ability of maturing oocytes to reach the morula/blastocyst stage after artificial (parthenogenetic) activation. It was shown that 51.8% of the oocytes reached stage MII. After artificial activation, 52.3% of the eggs were splitted and 33.6% of the oocytes (63.6% of the splitting embryos) reached the morula/blastocyst stage. The results show that the media of Life-Global, developed for the cultivation of human embryos, can be used for the maturation of porcine oocytes in vitro, which is confirmed by the successful use of eggs in experiments on parthenogenetic activation of embryos.
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