Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр животноводства – ВИЖ имени академика Л.К. Эрнста»
THE USE OF ISSR MARKERS FOR CHARACTERIZATION OF GENETIC DIFFERENTIATION OF CATTLE BREEDS
- Номер: No 3
- Страницы: 91-97
Журнал: Problemy biologii productivnykh zhivotnykh - Problems of Productive Animal Biology
Аннотация:
ABSTRACT. To date, different DNA markers and methods have been developed and widely employed for the determination of genetic polymorphisms and evaluation of the genetic structure of farm animal populations. But despite the diversity of the developed methods, it is difficult to choose the most reliable and the least labor-consuming approach with easily interpretable results. One of the promising and reliable methods that meet the requirements of multilocus genotyping is the method of inter-simple sequence repeat (ISSR) markers. As ISSR markers in the current work, 6 microsatellites have been used as primers in polymerase chain reaction (PCR) to evaluate genetic differentiation of dairy breeds and non-specialized cattle (holsteinized Black-and-White, Estonian Red, Ayrshire, Kazakh Whiteheaded, Tagil native cattle, Yakutian cattle, zeboid cattle, 10 animals of each breed). According to obtained results, the most polymorphic spectra were obtained by (GAG)6C and (AGC)6G primers. The dendrogram built by ISSR-PCR evaluation of different cow breeds demonstrated the close link between discovered polymorphism of DNA fragments under study and the animals’ differentiation by trend of productivity (dairy or non-specialized). The presented data demonstrate that every cattle group has specific features in amplicon spectrum polymorphism revealed by the use of microsatellite sequences as PCR primers (ISSR-PCR markers).The obtained data suggest the possibility to use the approach of ISSR-PCR merkers for development of breed-specific test systems. These would improve the selection and breeding of animals with desirable phenotypes and help consolidate the groups of cattle with respect to their breed affiliation.
Литература:
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