Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр животноводства – ВИЖ имени академика Л.К. Эрнста»

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Recombinant follicle-stimulating hormone for induction of superovulation in cattle: сurrent state and perspectives (a review)

Bursakov S.A., Kovalchuk S.N., Popov D.V., Kosovsky G.Yu.

Center of Experimental Embryology and Reproductive Biotechnologies,

Moscow, Russian Federation.

Номер: 2
Страницы: 5-23

Журнал: Проблемы биологии продуктивных животных

Ключевые слова:

cattle, embryo transplantation, gene-engineering techniques, glycosylation, recombinant follicle-stimulating hormone

Аннотация:

ABSTRACT. An important element of embryo transfer technology in cattle is a hormonal stimulation of superovulation in donor cows, that is based on the use of follicle stimulating hormone (FSH) for simultaneous growth and maturation of multiple follicles. For elaboration of biologically valuable embryos, it needs to enforce all the necessary conditions, but to date it has not yet formed a unified form of the production and use of FSH. A variety of methods for production of recombinant FSH for animal husbandry has increased significantly over the last 30 years, but so far failed to develop a common standard, that is consistent with the existing requirements to be included in protocol for induction of superovulation in cattle on a permanent basis and for obtaining the expected number of embryos per donor animal. The greatest success is possible in the production of recombinant FSH (rFSH), homogeneous in composition and close to their natural counterparts, but which has new features to increase the biological effectiveness and economic viability. Expenses for the purchase of gonadotropins are of paramount importance, since they are the most expensive part in the performance of embryo transfer. The use of rFSH may improve economic efficiency, predictability in embryo transplantation and efficacy to get oocytes suitable for fertilization. Besides, the application of a new generation of hormone analog will reduce side effects associated with its intensive utilization for the selected high yielding donor cows. The review presents systematic data on existing expression systems for recombinant FSH production and examples of molecular modeling using protein engineering technologies to create highly effective forms of hormone with desired properties.

Литература:

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