Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр животноводства – ВИЖ имени академика Л.К. Эрнста»
ABSTRACT. The aim of the study is to investigate the ability of the zygotes to develop to the blastocyst stage after microinjection into their pronuclei of a mixture of plasmids for expressing the components of CRISPR/Cas9 system and cultivation on commercial media. Cell donors were female C57BL6/CBA mice aged 6-9 weeks. As a manipulation medium, the Global with Hepes® commercial medium was used with the addition of 10% whey protein substitute, and as a culture medium – Global® with the addition of 10% whey protein substitute. As a result, data have been obtained that indicate that the media studied can be successfully used to produce blastocysts. During cultivation after microinjection, 84.5% of embryos entered the development within a day, reaching 52% before the stage of compact morula, of which up to the stage of blastocyst – 92%. It was concluded that the yield of blastocysts from the number of micro-injected zygotes at the level of 44% is sufficient to carry out further work on obtaining genomic-edited animals that produce recombinant proteins with milk.
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