Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр животноводства – ВИЖ имени академика Л.К. Эрнста»
ABSTRACT. The article is a mini-review of the new (opened, mostly, in the last 10 years) reactions catalyzed by porcine pancreatic lipase. In comparison with lipases from the another sources, porcine pancreatic lipase is a more stable and active enzyme in esterification and transesterification reactions in aqueous-organic media. The action of the enzyme is not limited by traditional substrates − esters of fatty acids. It was shown that this enzyme is able to catalyze a sufficiently wide range of completely different reactions - the formation of carbon-carbon, carbon-heteroatom, heteroatom-heteroatom bonds. There were described the following reactions: polymerization of2,2-dimethyltrimethylene carbonate; Michael addition, on the base of which were synthesized a lot of warfarin derivatives; aldol condensation, Henry reaction, stereo-selective hydrolysis and synthesis, which permit relatively simple production of enantiomers for the synthesis of many pharmacologically active compounds; one-step catalysis of some complex reactions, including one-pot synthesis of tetrahydrochromene derivatives at room temperature. The discovery of new catalytic capabilities of porcine pancreatic lipase broadened the range of biotechnological applications of this enzyme.
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