Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр животноводства – ВИЖ имени академика Л.К. Эрнста»
ABSTRACT. The protective properties of lithium ascorbate (AL) were studied in vivo in three experiments conducted on 5 groups of two-month-old Wistar rats under conditions of transport and immobilization stress: group I - stress model, control (rats receive water); II - model of stress, 120 mg AL /kg BW; III - model of stress, 60 mg AL/kg; IV - model of stress, 30 mg AL/kg; V - intact rats (without stress model). In groups II-IV, AL was administered once daily 2 hours after morning feeding in the form of an aqueous solution hrough the esophageal tube, the volume of administration was 1.5 ml. In the immobilization stress test, the animals were hung for 24 hours, then blood probe was taken, decapitation was performed, the abdominal cavity was opened and the number of ulcers on the inner surface of the stomach was counted. In the transport stress test, the rats were placed in cells fixed on a laboratory joker for 240 minutes. At the end of the test, the activity of alanine aminotransferase, aspartate aminotransferase and the creatinine content were determined in blood serum. In vitro studies used 7-8-day cultures of granular neurons of the cerebellum, subjected to the cytotoxic effect of glutamate (100 mM). In experiments in vivo, the level of adrenaline and norepinephrine in the blood serum under the influence of AL decreased vs control (P <0.05) against the background of a constant pool of eosinophils, and the parameters of adaptation in immobilization and transport stress tests improved. The pronounced neuroprotective effect of AL under the conditions of cytotoxic action of glutamate in vitro in the whole range of concentrations (0.1-1.0 mM) is shown. The obtained data indicate the expressed adaptogenic properties of lithium ascorbate under stress in vivoand in vitro and the close connection between stress and neurodestructive processes at the cellular level. The results of the research expand knowledge of the neuroprotective potential of lithium preparations, which was shown earlier in experiments on brain cell cultures.
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