Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр животноводства – ВИЖ имени академика Л.К. Эрнста»
ABSTRACT. On the basis of previously obtained pβLgGCSF plasmid containing the coding sequence of the gene human granulocyte-colony stimulating factor (G-CSF) under the control of regulatory elements of the bovine β-lactoglobulin gene and pGEMTcmvEGFP plasmid, containing the gene for EGFP (modified green fluorescent protein) under the promoter cmv (cytomegalovirus) and the bovine growth hormone polyadenylation site (BGH рolyA site), recombinant plasmid pβLgGCSFcmvEGFP was designed.
From the pβLgGCSF plasmid on the XbaI site a βLgGCSF fragment was cut out, which after purification was cloned into a vector pBluescript II SK(-) hydrolyzed on the same site. From pGEMTcmvEGFP plasmid with BsmBI (Esp3I) restrictases the fragment cmv-EGFP-BGH PolyA site (cmvEGFP) was cut and purified, which was cloned in plasmid pβLgGCS on the site NotI. The βLgGCSFcmvEGFP construction was cut with ClaI restrictase and used to study the integration of transgene at different stages of ontogenesis of laboratory and farm animals.
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