Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр животноводства – ВИЖ имени академика Л.К. Эрнста»
ABSTRACT. Bovine anaplasmosis is a transmissive infectious disease caused by rickettsia Anaplasma marginale (order Riсkettsiales, family Anaplasmatacеa). The forehanded diagnostics play a crucial role in prevention of bovine anaplasmosis distribution. A real-time polymerase chain reaction method (real-time PCR) is currently used for molecular diagnostics of infectious diseases due to its high sensitivity and specificity as well as the ability to simultaneously determine the level of bacterial and viral load. The aim of the study was to test a modified method based on real-time PCR and evaluate the parasitemia level in A. marginale-infected cattle. The infected animals were determined by the real-time PCR assay with a pair of species-specific primers and TaqMan probe to the A. marginale msp4 gene, and the parasitemia level has been estimated to be in the range from3,01×104 to 5,42×105 per ml of blood. The discribed method of estimating the parasitemia level in cases of bovine anaplasmosis could be used for determining the effectiveness of treatment of the infected animals with antirickettsial drugs and the efficacy of vaccines against bovine anaplasmosis.
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