Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр животноводства – ВИЖ имени академика Л.К. Эрнста»
1Ostrenko K.S., 1Galochkin V.A., 1Galochkin V.P., 2Gromova O.A,
2Pronin A.V., 3Torshin I.Yu.
1Institute of Animal Physiology, Biochemistry and Nutrition, Borovsk, Kaluga oblast;
2 Ivanovo State Medical Academy, Ivanovo; 3 Moscow Institute of Physics and Technology
(State University), Dolgoprudny, Russian Federation
ABSTRACT. The aim was to study the negative effects of lithium ascorbate (LA) on single-time and long-term oral administration for the management of stress conditions in laboratory animals, to determine toxic doses and to develop methods for reducing negative effects. Target organs affected by prolonged use of toxic doses and the effect of LA on metabolism were studied in Wistar rats. It was found that the LD50 of LA is 6.334 g/kg body weight. Substance refers to compounds of 5th toxicity class, i.e. practically non-toxic. The pathoanatomical picture of the fallen animals shows the symptoms of acute poisoning and the onset of death from cardiopulmonary insufficiency and atrophy of the excretory system. The use of LA in a dose of 1/3 LD50 (2.11 g/kg) had a moderately negative effect on rats. The coefficient of cumulation is 14.8, which indicates a low cumulative effect and low toxicity. With multiple doses of LA exceeding LD50 (subchronic toxicity), some of the experimental animals were adapted and maintained viability. Histological examination data indicate that against the background of the preservation of the general histostructure, the functional overstrain of the neuroendocrine apparatus and, to a lesser extent, the organs of excretion and reproduction occurred. Since the animals were killed 2 weeks after the end of the LA administration, during this period, a partial restoration of the functional activity of the brain, liver, kidneys, testes and ovaries appeared. This is indicated by moderate hyperplasia of the cellular elements of the proliferative zone of the adenohypophysis. Due to the individual reaction of animals to LA, the severity of the described changes in animals of the same group was somewhat different, while LA activates the neuroendocrine system more strongly in females than in males.
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