Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр животноводства – ВИЖ имени академика Л.К. Эрнста»
ABSTRACT. The aim was to study the effect of serotonin (5-HT) and cholecystokinin (CCK) on the feeding behavior of carp Cyprinus carpio in conditions of combined effects of light deprivation and biogenic metals. To study of feeding behavior, juvenile carp were placed in a 40 l chamber of transparent plexiglas with perforations, which was installed at the back wall of the aquarium. The illumination mode in the control groups was 6:18, in the experimental groups - 0:24. In two control and two experimental aquariums, 1 μmol of ZnSO4 • 7H2O was added, and in the other four - 1 μmol CuSO4 • 5H2O by the calculation for metal. At the front wall of the aquarium, food was placed (frozen larvae of chironomids, weight 9.5 mg). When the front wall of the chamber was raised, the fish could leave the chamber to search for and consume the food. We recorded the time of finding the fish in the starting compartment after lifting the front wall of the chamber, the time necessary for the fish to reach the feed, inversely proportional to the rate of the feeding reaction, and the amount of food consumed. 0.1 ml of Ringer's solution for cold-blooded animals was injected intraperitoneally into the fish of the control group 1 hour prior to the experiments, an equal amount of serotonin hydrochloride (5-HT), at a dose of 10 μ/g of body weight or cholecystokinin (CCK) at a dose of 100 ng/g of body weight. It was established that under the conditions of light deprivation, the effects of 5-HT and CCK decrease. The degree of reduction depends on the presence of Zn and Cu in water. After 1 hour under the influence of 5-HT, the time of the residence time of fish in the chamber decreases in the presence of Zn by 25, Cu - by 17% compared to fish under variable illumination conditions. The time needed to reach the fodder spot is reduced by 80 and 94%, the food intake is increased by 22 and 650%, respectively, compared to fish that are under variable illumination conditions. Under the influence of CCK combined with light deprivation after 1 h, the time of the residence time of fish in the chamber decreases in the presence of Zn by 33, Cu - by 22%. The time needed to reach the fodder spot is reduced by 75 and 53%, food intake is increased by 132 and 25%, respectively, compared to fish that are under variable illumination conditions. The mechanisms of the combined effect of 5-HT, CCC, light deprivation and biogenic metals on the feeding behavior of fish are discussed.
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