Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр животноводства – ВИЖ имени академика Л.К. Эрнста»
ABSTRACT. Disposal of phenolic water in reservoirs causes a significant change in the regime of biogenic elements, level of oxygen and carbon dioxide; phenol also exerts a negative influence on aquatic organisms. The aim was to study in vitro the effects of phenol and its derivatives on the activity in digestive tract of tripsin- and chymotrypsin peptidases in different fish species. The subjects were bream Abramis brama (L.), white breamBlicca bjoerkna (L.), roach Rutilus rutilus (L.), river perch Perca fluviatilis L. from Rybinsk reservoir and pond carp Carassius carassius (L.). Caseinlytic activity (predominantly trypsin activity) and hemoglobin-lytic activity (predominantly chymotrypsin activity) were evaluated in intestinal mucosa and chyme during incubation of homogenates in the presence of phenol and its derivatives (4-chlorophenol, 4-nitrophenol, 2,4-dinitrophenol) at a concentration of 1 mM. Phenol and its derivatives induced a significant decrease in activity and casein- and hemoglobinlytic peptidases of the intestinal mucosa and chyme from different fish species. The degree of reduction of enzyme activity depends on the location and type of fish peptidases. Phenol and its derivatives caused a significant decrease in activity and casein- and hemoglobin-lytic peptidases of the intestinal mucosa and chyme in different fish species. The degree of decrease in enzyme activity depends on the localization of peptidases and on fish species.
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