Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр животноводства – ВИЖ имени академика Л.К. Эрнста»
Three trials were performed on newborn calves and milky calves, with parenteral injection of dipeptide thymogen (T, glu-trp), aimed to stimulate absorption of colostral immune globulins and formation of nonspecific resistance. In trial I, 5 calves were injected T twofold at first hour and at 4-5 hours after birth, blood probes were taken at 1 and 10 days after birth. In trial II, the 20-30-days-calves were injected with a prolonged form of T twofold with interval 6 days at the dose 500 μg (group II, n=7), in group III (n=7) at 8 days before T injection, derinate (D, sodium desoxyribonucleinate) was injected at the dose of 500 mg. All calves of this trial were housed outdoor (cold method of growing). Third trial was performed according the same scheme as in trial II, the calves were weighted at the end of first and second month of trial. The blood probes were taken at 15 days after injection of T. In trial I in treatment group, the increased blood level of immune globulins was indicated (P<0.05 vs control). In trial II, any significant shifts in biochemical and morphological indices of blood were not indicated. In trial III after second injection of T, the increase was indicated in blood level of leucocytes (P<0.05), mainly at the expense of polymorphonuclear neutrophils (P< 0.05), and in bactericidic activity of serum (P<0.05). The action of D combinated with T, was accompanied by tendency to increases in number of erhytrocytes and leucocytes, total number of neutrophyles, and in blood hemoglobin level. The amelioration of nonspecific resistance of calves in treatment groups was accompanied by increases in their live weight.
1. Asatiani V.S. Novye metody biokhimicheskoi fotometrii (Novel methods of biochemical methods of photometry). Moscow, 1965, 543 p.
2. Belokrylov G.A., Popova O.Ya., Sorochinskaya E.I., Derevina O.M., Korovin R.N. Doklady Rossiiskoi Academii Sel'skokhozyaistvennykh Nauk - Russian Agricultural Sciences, 2001, 2, 51-52.
3. Goldstein A.L., Cohen G.H., Zatz M.M. Purification and biological activity of thymosin, a hormone of thymus gland. Proc. Natl. Acad. Sci. USA., 1972, 69: 1800-1803.
4. Karput' I.M., Pivovar L.M. Vestnik AN BSSR, ser. s.-kh. nauki - Herald of Belarusian Academy of Sciences, Ser. Agric. Sci., 1982, 4: 108-110.
5. Kharitonov L.V., Kuznetsov I.L., Pron'kina E.A., Velikanov V.I. Trudy VNIIFBiP (Proc. Inst. Anim. Physiol. Biochem. Nutr.), 2002, 41: 83-96.
6. Kharitonov L.V., Matveev V.A., Velikanov V.I., Pron'kin D.E. Materialy 3-i mezhd. konf.: Aktual'nye problemy biologii v zhivotnovodstve (Proc. 3rd Intern. Conf.: Urgent biological problems in animal husbandry), Borovsk, 2001: 177-188.
7. Kharitonov L.V., Morozov A.N., Kharitonova O.V. Problemy biologii produktivnykh zhivotnykh - Problems of Productive Animal Biology, 2012, 2: 42-48.
8. Khavinson V.Kh., Anisimov V.N. Peptidnye bioregulyatory i starenie (Peptide bioregulators and aging). St. Petersburg: Nauka, 2003, 223 p.
9. Khavinson V.Kh., Kvetnoi I.M., Ashmarin I.P. Uspekhi sovremennoi biologii - Advances in Contemporary Biology, 2002, 122(4): 554-570.
10. Kondrakhin I.P., Kurilov N.V., Malakhov A.G. et al. Klinicheskaya laboratornaya diagnostika v veterinarii. Spravochnoe izdanie (Clinical laboratory diagnostics in veterinary medicine). Moscow: Agropromizdat, 1985, 287 p.
11. Kovalenko Ya. R. Vestnik selskokhozyaistvennoi nauki – Herald of Agricultural Science, 1979, 2: 50-58.
12. Metody biokhimicheskogo analiza. Spravochnoe posobie (Methods of biochemical analysis: a manual, Ed. B.D. Kal'nitskii). Borovsk, 1997, 357 p.
13. Miller J.F. Immunologic function of thymus. Lancet, 1961, 2: 748-749.
14. Morozov V.G., Khavinson V.K. Natural and synthetic thymus peptides as therapeutics for immune dysfunction. Int. Immunopharm., 1997, 19: 501-505.
15. Morozov V.G., Khavinson V.Kh., Malinin V.V. Peptidnye timomimetiki (Peptide thymomimetics). St. Petersburg: Nauka, 2000, 158 p.
16. Smirnov V.S. Timogen v zhivotnovodstve i veterinarii (Thymogene in animal science and veterinary medicine). St. Petersburg, 2005, 36 p.
17. Velikanov V.I., Shumov I.S., Maslova M.A., Kharitonov L.V. Materialy 18-i mezhd. konf.: Novye farmakologicheskie sredstva v veterinarii (Proc.18th Intern. Conf.: Novel pharmacological materials in veterinary medicine). St. Petersburg, 2006: 49-50.
18. Vorob'ev A.A., Lyashchenko V.A. Zhurnal mikrobiologii, epidemiologii I immunologii – Journal of Microbiology, Epidemiology and Immunology , 1995, 6: 105-111.