Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр животноводства – ВИЖ имени академика Л.К. Эрнста»
ABSTRACT. The aim of the study was to investigate the activity of adenylate cyclase (AC) in lymphocytes isolated from peripheral blood of cows exposed to a common external action of γ-radiation at a dose of 1 Gy/hr (137Cs) source with an energy of gamma rays of 0.67 MeV). The trial was performed on 29 Black-and-White cows, divided into three groups. Group I (n = 9) was control, groups II and III, 10 cows each, were exposed to the total external γ-radiation in the half-lethal (LD50/30) - 3.5 Gy and lethal (LD100/30) − 6 Gy doses, respectively. Acute radiation syndrome developed in group II of secondary, and in group III of 3rd degrees of severity. The level of basal and stimulated prostaglandin E1 AC activity increased in both treatment groups as early as 1 day after irradiation. The basal activity in group II during 10 days maintained at a level exceeding by 3-4 times the control (P<0.05), and in group 3 by 2-fold (P<0.05) for 5 days. Prostaglandin E1-stimulated AC activity exceeded the initial level in these periods in group II by 3-7 times (P<0.05), and in group III by 3-6 times (P<0.05). The level of basal and stimulated activity of AC in group III during 10 days was below initial level, and in group III it remained high during this period. AC activity in lymphocytes of cows exposed to a lethal dose, increased during initial and latent periods, and decreased during the height of radiation damage. In general, the total external influence action of γ-radiation in the half-lethal dose causes in cows an increase in basal and prostaglandin E1 stimulated AC activity in lymphocytes in all investigated periods after exposure (30 days), indicating the post-radiation activation of adenylate cyclase signaling system in lymphocytes in all periods after radiation exposure. Lethal dose of irradiation leads to activation of lymphocytes in the system during the primary reaction and the latency period, and during the height of the radiation damage it leads to its inhibition.
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