Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр животноводства – ВИЖ имени академика Л.К. Эрнста»
Galochkina V.P., Agafonovа A.V., Dudin V.I.
Institute of Physiology, Biochemistry and Animal Nutrition,
Borovsk Kaluga oblast, Russian Federation
ABSTRACT. The experiment was conducted on three groups of Black-and-White cows, 3 cows each, in the first third of lactation with daily milk yield 44.3±1.5; 35.0±0.6 and 26.3±3.2 kg (M±SD) in I, II and III groups respectively. Thiamine, folacin, pyruvate and acetaldehyde were determined in milk, blood plasma and urine, the activity of pyruvate carboxylase (PC) and lactate dehydrogenase (LDH) was determined in the presence of NAD. In high producing animals, the excretion of thiamine and folacin in milk was raised compared to low producing cows, and despite of compensatory decrease of urinary excretion, a deficiency of these vitamins was created in the organism. The low ratio of PC/LDH in groups (0.037-0.127) shows that the predominant use of pyruvate was in the synthesis of glucose and in the metabolization via oxaloacetate in the Krebs cycle. There was a lack of thiamine and folacin in all groups, especially in high producing cows. Thiamine deficiency reduced pyruvate dehydrogenase activity and increased output of acetaldehyde with urine. Due to the low activity of PC and LDH, there was an accumulation of pyruvate in blood serum and increased urinary excretion of pyruvate. These results suggest that in the experimental conditions with increasing milk production, the flux of pyruvate utilization shifted in the direction of transamination reactions. This shifts in pyruvate metabolism facilitated increased protein synthesis in the liver and mammary gland.
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