Всероссийский научно-исследовательский институт физиологии, биохимии и питания животных – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр животноводства – ВИЖ имени академика Л.К. Эрнста»
The activity of proteinases and glycosidases of digestive tract in sterlet Acipenser ruthenus (L.), grown in aquaculture conditions as well as temperature characteristics of proteinases were investigated. In hungry fish pepsin-like activity was 6.6–8.5 folds lower than those in feeding fish. The activity of trypsin-like proteinases was approximately order of magnitude higher than glycosidase activity. The character of the proximal-distal gradient of enzyme activity in sterlet is close to that of ichthyophages from natural waters. The values of the temperature optimum of pepsin-like proteinase of stomach mucosa correspond to 40ºC, trypsin-like proteases of the intestine – 50ºC, chymotrypsin-like proteases – 60ºC. In the temperature range for fish feeding, trypsin-like proteinases of chyme have higher values of their relative activity (35-80%) compared to those of the mucosa (5-50%). At this temperature range, Eact value of stomach mucosa is 2.5 kcal/mol. Eact value of intestinal mucosa and chyme does vary depending on temperature. But in the zone of 0-20ºC they are significantly lower than in the zone of higher temperature.
1. Anson M. The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin. J. Gen. Physiol., 1938, 22: 79-83.
2. Bednyakov D.A., Nevalennaya L.A., Novinskii V.Yu. Vestnik AGTU – Herald Astraсhan State Technical Univers., Ser. Fish Industry, 2011, 2: 74-77.
3. Bednyakov D.A., Nevalennyi A.N. Vestnik AGTU – Herald Astrakhan State Technical Univers., Ser. Fish Industry, 2013, 1: 124-128.
4. Golovanova I.L., Kuz’mina V.V., Gobzhelian T.E., Pavlov D.F., Chuiko G.M. In vitro effects of cadmium and DDVP (Dichlorvos) on intestinal carbohydrase and protease activities in frashwater teleosts. Comp. Physiol. Biochem., 1999, 122C (1): 21-25.
5. Kishimura H., Tokuda Y., Klomklao S., Benjakul S., Ando S. Enzymatic characteristics of trypsin from pyloric ceca of spotted mackerel (Scomber australasicus). J. Food Biochem., 2006, 30: 466-477.
6. Korostelev S.G., Nevalennyi A.N., Levchenko O.E. Biologiya morya – Marine Biology, 2005, 31(3): 221-224.
7. Kuz’mina V.V., Kuz’mina E.G. Voprosy ikhtyologii - Problems of Ichthiology, 1991, 31(2): 306-313.
8. Kuz’mina V.V. Doklady Rossiiskoi Akademii Nauk - Proc. Russ. Acad. Sci., 1992, 326(4): 749-752.
9. Kuz’mina V.V., Golovanova I. L., Kovalenko E.E. Separate and combined effects of cadmium, temperature and pH on digestive enzymes in thries fresh water teleosts. Env. Contam. Toxicol., 2002, 69(2): 302-308.
10. Kuz’mina V.V. Fiziologo-biokhimicheskie osnovy ekzotrofii ryb (Physiological and biochemical bases of fish’s exotrophy). Moscow: Nauka, 2005, 300 p.
11. Kuz’mina V.V. Fiziologiya pitaniya ryb. Vliyanie vneshnikh i vnutrennikh faktorov (Physiology of fish nutrition. Effects of outer and inner factors). Boroc: Institute of Inland Waters Biology RAS, 2008, 276 p.
12. Kuz’mina V.V., Skvortsova E.G., Shalygin M.V. Zhurnal evolyutsionnoi biokhimii I fisiologii - J. Evol. Biochem. Physiol., 2008, 44(5): 482-487.
13. Kuz’mina V.V., Ushakova N.V. The dependence on temperature and pH of the effects of zinc and copper on proteolytic activities of the digestive tract mucosa in piscivorous fish and their potential preys. Fish Physiol. Biochem., 2010, 36(3): 787-795.
14. Kuz’mina V.V., Skvortsova E.G., Shalygin M.V. Problemy biologii productivnykh zhivotnykh - Problems of Productive Animal Biology, 2012, 3: 22-29.
15. Kuz’mina V.V., Skvortsova E.G., Shalygin M.V. Problemy biologii productivnykh zhivotnykh - Problems of Productive Animal Biology, 2012, 3: 52-59.
16. Munilla-Morán R., Saborido-Rey F. Digestive enzymes in marine species. I. Proteinase activities in gut from redfish (Sebastes mentella), seabream (Sparus aurata) and turbot (Scophthalmus maximus). Comp. Biochem. Physiol., 1996, 113B(2): 395-402.
17. Natalia Y., Hashim R., Ali A., Chong A. Characterization of digestive enzymes in acarnivorous ornamental fish, the Asian bony tongue Scleropages formosus (Osteoglossidae). Aquaculture, 2004, 233: 305-320.
18. Nevalennyi A. N., Tuktarov A. V., Bednyakov D. A. Funktsional’naya organizatsiya i adaptivnaya regulyatsiya protsessov pishchevareniya u ryb (Functional organization and adaptive control of digestive processes in fish). Astrakhan: Astraсhan State Technical University, 2003, 152 p.
19. Nevalennyi A.N., Bednyakov D.A., Novinskii V.Yu. Vestnik AGTU – Herald Astraсhan State Technical University, Ser. Fish Industry, 2011, 2: 93-98.
20. Nikol’skii G.V. Chastnaya ikhtiologiya (Particular ichthiology). Moscow: Sovetskaya nauka, 1954, 458 p.
21. Ostroumova I.N. Biologicheskie osnovy kormleniya ryb (Biological bases of fish nutrition). St. Petersburg: GosNIORKH (State Research Institute of River and Lake Industry), 2012, 564 p.
22. Pavlisko A., Rial A., de Vecchi S., Coppes Z. Properties of pepsin and trypsin isolated from the digestive tract of Parona signata “Palometa”. J. Food Biochem., 1997, 21: 289-308.
23. Ponomarev V.I. Ekologiya -Ecology, 1995, 1: 86-89.
24. Sabapathy U., Teo L.-H. Some properties of the intestinal proteases of the rabbitfish, Siganus canaliculatus (Park). Fish Physiol. Biochem., 1995, 14(3): 215-221.
25. Shalygin M. V. Rol’ proteinaz ob’’ektov pitaniya i enteral’noi mikrobioty v temperaturnykh adaptatsiyakh pishchevaritel’noi sistemy ryb raznykh ekologicheskikh grupp (Role of nutritive objects proteinases and enteral microbiota in temperature adaptations of digestive system in fishes of various ecological groups). Extended Abstract of Diss., Borok, Institute of Inland Waters Biology, 2013, 22 p.
26. Shcherbina M.A., Gamygin E.A. Kormlenie ryb v presnovodnoi akvakul’ture (Nutrition of fishes in freshwater aquaculture). Moscow: Inst. Fish Industry and Oceanography, 2006, 360 p.
27. Ugolev A.M., Iezuitova N.N. Issledovanie pishchevaritel’nogo apparata u cheloveka. Obzor sovremennykh metodov (Study of digestive apparatus in human. Review of modern methods). Leningrad: Nauka, 1969: 169-173.
28. Ugolev A.M., Kuz’mina V.V. Pishchevaritel’nye protsessy i adaptatsii u ryb (Digestive processes and adaptations in fishes). St. Petersburg: Gidrometeoizdat, 1993, 238 p.
29. Ushakova N.V., Kuz’mina V.V. Voprosy ikhtyologii - Problems of Ichthiology, 2010, 50(4): 554-560.
30. Vysotskaya R.U., Nemova N.N. Lizosomy i lizosomal’nye fermenty ryb (Lisosomes and lisosomal enzymes of fishes). Moscow: Nauka, 2008, 284 p.